SÉMINAIRE DU 7 JUIN 2022:

This seminar will host Gertjan Medema, Yvon Maday and Laurent Moulin, who will present their researches about sewage surveillance during Covid-19.

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Location:

Laboratoire Jacques-Louis Lions, Sorbonne Université, Campus Pierre et Marie Curie salle 15-16-309 (barre 15-16, 3ème étage, porte 09) .(map)

Program:

10h30-11h15 : Gertjan Medema, (KWR and Delft University of Technology)

Capturing the SARS-CoV-2 infection pyramid within the municipality of Rotterdam using longitudinal sewage surveillance.

11h15-11h30 : Questions

11h30-12h15: Yvon Maday (Sorbonne Université) – Laurent Moulin (Eau de Paris)

A national wastewater surveillance system in France. The Obepine project : a tool to support public authorities during the Covid crisis.

12h15-12h30: Questions


List of participants

 

More details:

 

Gertjan Medema (presentation)

Capturing the SARS-CoV-2 infection pyramid within the municipality of Rotterdam using longitudinal sewage surveillance

Abstract

Sewage surveillance was implemented along with surveillance of cases as two parts of the surveillance pyramid in neighborhoods in the city of Rotterdam. There are still many unknowns about the correlation between levels of SARS-CoV-2 in sewage and reported SARS-CoV-2 positive tests is prone to bias by variability in both wastewater and testing data. Here, we have performed a longitudinal study which allowed us to investigated these biases and to model SARS-CoV-2 circulation based on the combination of wastewater and testing data within Rotterdam, the Netherlands.

Sewage samples were collected three times a week, from September 2020 to November 2021, from nine neighborhoods within the Rotterdam Rijnmond area. Normalized SARS-CoV-2 concentrations were compared with reported SARS-CoV-2 positive tests and test delay and intensity data, using zip codes to match the populations.

Using high resolution sampling, careful normalization of the wastewater concentrations as well as ‘normalization’ of the reported SARS-CoV-2 positive tests for testing delay and intensity, we were able to model the number of reported positive SARS-CoV-2 positive tests based on sewage data, and up- and downward trends in both surveillance systems coincided. Hence, ‘Eearly warning’ of wastewater surveillance was shown to be is governed by testing delay and the observed incidence of clinical testing was biased by testing intensity. The observed relation between wastewater and case data was not independent of the dominant SARS-CoV-2 variant of concern and levels of vaccination.

Sewage surveillance provides an objective measurement of SARS-CoV-2 circulation in the population that is independent of testing intensity and delays, unlike case surveillance. Complementing case surveillance with sewage surveillance will help public health authorities to verify trends and differences in incidence in different city areas.

Yvon Maday – Laurent Moulin

A national wastewater surveillance system in France. The Obepine project : a tool to support public authorities during the Covid crisis

Abstract

The Obépine project (OBservatoire EPIdémiologique daNs les Eaux usées) has brought together research teams with a variety of skills (virology, mathematics, hydrology, infectiology) to evaluate the relevance of a quantification for SARS-CoV-2 genomes and its variants in wastewater, to provide a new element for monitoring the COVD-19 epidemic. We have demonstrated the relevance of this strategy, which aims at quantifying the viral genome at the entrance of wastewater treatment plants thanks to the use of sensitive, quantifiable, and reproducible molecular techniques associated with an original mathematical model.

Since spring 2020, Obépine has been monitoring wastewater treatment plants at the national level, on a bi-weekly basis, in metropolitan and overseas France reaching mid 2021, some 200 treatment plants (representing about 40% of the French population).

This presentation summarizes the key steps in the construction of the Obépine project. (website)

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